| 1. | Expression was induced by adding iptg to a final concentration of imm and incubation with shaking at 30 for 3 h
以2 %比例接种入新鲜mg培养基，培养至对数中期，加入iptg至终浓度为1mm ，在温度为30的条件下诱导表达3小时。 |
| 2. | The different bivalent cations with the same final concentration were put into the dnaase reacting system for 15 minute at ph5 . 4 , at 41 , then dnaase ' s activity unit was detected
在dna酶的酶促反应体系中加入终浓度相同的二价金属阳离子41下、 phs 4准确反应15分钟后测定酶的活力单位。 |
| 3. | The optimum precipitation conditions of jujube date polysaccharide were obtained as follows : the extracting liquid was concentrated 4 times , adding 4 times volume ethanol and making the final concentration of alcohol 80 %
摘要本文研究了红枣多糖的沉淀条件，并对所提取的红枣多糖粗品的抗氧化作用进行了探讨。 |
| 4. | Medium experiments were arranged under uniform design , and then an optimum medium was got accordingly . the culture liquid was centrifugalized at 3 , 500r / min for 30min , then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others
通过硫酸铵分级沉淀、 deaesephadexa - 50阴离子交换凝胶层析和sephadexg - 75凝胶柱层析对发酵液进行分离和纯化，并得到电泳纯的酶。 |
| 5. | Expression and purification of recombinant rtas the expression plasmid pkk223 . 3 - rta was introduced into e . coli jm 109 by cacl2 - mediated method . the cells were grown until an optical density at 600 nm reached 0 . 6 . expression was induced in the presence of iptg ( final concentration 1 mm ) and incubated at30 for 3h )
以mtt法分别测定纯化后的rta与rta一yqrl蛋白对体外培养的wish (人羊膜上皮细胞) 、 skov3 (人卵巢癌细胞) 、和hela (人宫颈癌细胞)的毒性作用。 |
| 6. | A does - dependent study of the aba - induced oxidative burst was observed , in which both the magnitude and rate of dye quenching was directly related to the concentration of aba . and the treatment of the guard cells with catalase of 100 units / ml nearly completely obliterated the quenching reaction induced by 10 | amol / l aba ( final concentration )
因此， aba可以诱导气孔保卫细胞产生h2o2 ，其产生的强度和速度与aba的浓度直接有关，并且推测：这种h2o2产生可能是aba诱导气孔关闭过程中信号转导链的一个中间成分。 |
| 7. | Furthermore , when 10 imo il h2o2 ( final concentration ) was added to the suspension after above treatment caused little reduction in hpts fluorescence . the results obtained by laser scanning confocal microscopy suggested that addition of exogenous aba resulted in a rapid decrease in fluorescence in most cellular compartments of the guard cells
维生素c可部分逆转低浓度h2o2 ( 10 - 5 )所诱导的气孔关闭过程：并且10 - 6mol l的dpi和103u ml的过氧化氢酶( cat )在一定程度上也逆转了aba诱导张开气孔的关闭。 |
| 8. | The development of healthy concentrates includes various steps : 1 . start with plants that are organically grown , high in nutritional value ; 2 . harvest the plants when they reach their own peak nutrient levels ; 3 . harvest and process within hours before the nutrients start to degrade ; 4 . final concentration process locks in the nutrients
有益浓缩素之生产过程有数个步骤： 1 .以有机方法种植高养分的植物； 2 .于植物营养最高时进行收割； 3 .于收割后数小时内处理原料，避免营养流失； 4 .进行最后浓缩工序以保留原料中的营养。 |
| 9. | It was estimated that the yield of the e2 fusion protein in culture supernatant of recombinant p . pastoris could be reached 0 . 34g / l . the optimal expression conditions were explored for the ph , aeration rate , final concentration of methanol and kinds of growth media . we conclude that the higher quantity of e2 protein can be gained at ph4 . 0 - 6 . 0 , 1 % methanol as the final concentration , mm growth media and high aeration rate
通过对表达条件如ph值、通气量、诱导甲醇终浓度、培养基的选择等条件的探索，可确定猪瘟病毒hl - ly株e2基因表达的理想条件为：最适ph值在4 . 0 6 . 0之间，最适培养基为mm培养基，最佳诱导的甲醇终浓度为1 ，加大通气量时表达量较好。 |
| 10. | Klac gene was ligated to the pet - 30a ( + ) vector for expression . then the recombinant plasmid petlac4 was transformed into e . coli bl21 . the positive transformants were induced at different temperature for three hours by iptg with the final concentration of lmm . sds - page analysis and lactase activity assay showed that klac gene was expressed in e . coli , and that the expression level at 28 was much higher than that at 37
表达产物的sds - page分析和酶活性测定表明， klac基因在大肠杆菌中获得活性表达，其中低温条件28下的表达水平明显高于37 ， 28诱导的细胞经超声波破碎所测酶活力为0 . 475u / ml ， 37仅为0 . 134u / ml 。 |
